BPS3151 Study Guide - Quiz Guide: T7 Rna Polymerase, Absorbance

• School: University of Ottawa
• Department: Biopharmaceutical sciences
• Course: BPS3151
• Professor: Luc Poitras

Document Summary

Protein amounts obtained at the different purification steps based on the 280 of the recombinant t7 rna polymerase from the laboratory class 5 assignment. [((0. 337) / (42976. 8 m-1cm-1) * (0. 05 cm)] * (360 aa * 110 g/mol) 0. 0155 g/ l (protein concentration) * (total volume of the fraction) (0. 0155 g/ l) * (50 l) [((a280) / ( )) * (l)] * (e. coli protein weight) [((0. 005) / (140260 m-1cm-1) * (0. 05 cm)] * (360 aa * 110 g/mol) Total amount of protein (protein concentration) * (total volume of the fraction) (0. 0000706 g/ l) * (1500 l) Loading sequence on sds-page gel for western analysis of protein fraction aliquots. Input control, 10 g or a maximum of 12. 5 l. Flowthrough control, 10 g or a maximum of 12. 5 l. 4 wash 1 control, 1 g or a maximum of 12. 5 l. 5 wash 2 control, 1 g or a maximum of 12. 5 l.