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Experiment 1.docx

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Department
Chemistry
Course
CHM2123
Professor
All Professors
Semester
Spring

Description
UniversityofOttawaOrganicChemistryLabIICHM2123SectionBExperiment1SeparationandPurificationofNaturalProductsForLuisVillegasthDueSeptember242013FromAnchalJoshi7449898LabPartnerViNguyen7390183IntroductionThepurposeofthisexperimentwastousedifferenttechniquestoextractandpurifycertainproductsinachemicalreactionProcessessuchasextractionrecrystallizationandthinlayerchromatographyTLCweredoneinordertoyieldacertainproductInpartAoftheexperimentextractionwasusedinordertoisolatethecaffeineinblacktealeavesandthenrecrystallizationmethodswereusedtopurifytheextractedproductInpartBextractionwasdoneinordertoisolatethepigmentsinspinachleavesaswellasTLCtoseparatethepigmentsanddeterminewhichpigmentsarepresentintheleavesPartAExtractionandPurificationofCaffeinefromBlackTeaTealeaveshavebeenknownforitsmedicinalpropertiesfromthecaffeineandtheactivityofpolyphenolsTheyarecomposedofpolyphenolssuchastanninscaffeinetheaflavincatechinandtheanineaswellasotherplantmaterialsuchaschlorophyllsandcelluloseCaffeineisaverycommonsubstancefoundincoffeeteasoftdrinkschocolateandmanyothergoodsItcaneasilybesynthesizesandisolatedfromnaturalsourcesThisexperimentillustratestheisolationofanaturallyoccurringproductfromaplantmaterialcaffeinefromblackteaFigure1PolyphenolspresentinteaCathechinTheaflavinTheanineCaffeine137trimethylxanthineisanalkaloidItisusedasstimulatorydrugforthecentralnervoussystemskeletalmusclesandrespiratoryandcardiacsystemsBlackteacontainsapproximately30to75mgofcaffeineper250mLcupLikeotheralkaloidssuchasmorphinecocaineandcodeinecaffeineisknowntohavehighlyaddictivepropertiesTheextractionofcaffeinecaneasilybedonewithhotwaterbutmanytanninsdissolveintotheaqueouslayerthereforeanorganicsolventsuchasdichloromethane
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