CHM 1321 Study Guide - Quiz Guide: Ethyl Acetate, Lab Report, Biphenyl

• School: University of Ottawa
• Department: Chemistry
• Course: CHM 1321
• Professor: Greg Flynn

Document Summary

The purpose of this lab was to discover the identity of unknown compounds through the use of tlc. Tlc, or thin layer chromatography, is a technique used to separate non-volatile mixtures. Tlc is commonly performed on a sheet of aluminum foil that is coated in an absorbent layer of silica known as the stationary phase. Draw three equally spaced tic marks on this line. The outside lanes should be at least 5 mm from each edge. Be sure to label the test tube: spot this sample solution onto both of the plates you prepared in step 2 on the sample lane and on the co-spot lane. To apply a spot, dip a capillary in the solution. Touch the end of the capillary to the tlc plate at the appropriate lane. You will see the solution expand on the plate into a small spot (about 2 mm wide). Wait a few seconds for the solvent to evaporate.