HSS 1101 Study Guide - Final Guide: Autoclave, Botulism, Pasteurization

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Hss1100: midterm 2- 30 mcq and 30 marks short answer. List all the five steps in virus replication (in the correct order). Live-attenuated vaccine, killed vaccine (or toxoid), recombinant vaccine, absorbed vaccine, conjugate vaccine. Autoclaving, pasteurization, ionizing radiation, nonionizing radiation, moist heat (5 points) Autoclaving - most common, no air involved, saturated (vapour) and dry (no droplets) steam because if its wet it soaks fabrics, impedes heat transfer and prolongs drying time. Pasteurization - heat below boiling point to destroy vegetative form of pathogen, usually for heat- sensitive liquids ionizing radiation - enough kinetic energy to liberate electrons from atoms (most uv, gamma) Non-ionizing radiation - does not have enough energy to ionize atoms (microwave) Moist heat - irreversible decontamination of proteins by coagulation. The order of resistance in nocosomial infections section: (5 points) bacterial spores and protozoan cysts --> mycobacteria --> fungi --> vegetative bacteria --> enveloped viruses.

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