BIO315H5 Final: LECTURE 23 – CELL CYCLE
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In order to enter into s phase, you must have 2 criteria: Lecture 23 cell cycle: dna replication must be extremely accurate to avoid mutations, dna replication must only occur once per cell cycle otherwise you will have extra dna which is not. Two steps in the initiation of dna replication: at this step you have the origins of replication which are sites along chromosomes where replication can be initiated. You have additional proteins being deposited at this site. This complex that forms at these origins is called the pre-rc. Ensures that replication is permitted only at these sites. When s-cdk turn on activity of helicases, causes dna to unwind and replication can be initiated. Both steps are controlled by cdks formation of pre-rc is inhibited by cdks and stimulated by apc/c, this ensures that pre-rc assembly only occurs during late mitosis and early g1.
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