BIOB10H3 Study Guide - Final Guide: Tubulin, Spindle Apparatus, Nocodazole

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11 Dec 2016
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Forms polymers by joining several protein subunits through weak & non-covalent bonds. Allo(cid:449) rapid asse(cid:373)(cid:271)ly & disasse(cid:373)(cid:271)ly (cid:894)as a key (cid:272)hara(cid:272)teristi(cid:272) for (cid:272)ytoskeleto(cid:374)"s fu(cid:374)(cid:272)tio(cid:374)(cid:895) Intracellular transport: allows movement of materials & organelles within cells. Cell motility & cell functions (e. g. phagocytosis) [cilia & flagella] The cytoskeleton is made up of 3 components: microtubules, microfilaments (actin filaments) Hollow, tubular structures found in almost all eukaryotic cells (25nm diameter) Tubulin: protein building blocks [has 2 forms of subunits] & tu(cid:271)uli(cid:374): very si(cid:373)ilar i(cid:374) stru(cid:272)ture & fit together (cid:448)ery (cid:449)ell form dimers = heterodimers. Tubulin (is a gtpase) has gtp that will be slowly hydrolyzed to gdp after polymerization occurs. Tubulin has stable gtp bound that does not get hydrolyzed / exchanged. & dou(cid:271)lets for(cid:373)i(cid:374)g a lo(cid:374)g poly(cid:373)er. Asy(cid:373)(cid:373)etri(cid:272): & -tubulin on different end gi(cid:448)es polarity (cid:894) -(cid:373)i(cid:374)us e(cid:374)d & -plus end) Polymerization addition of heterodimers to the -plus end.

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