BIO230H1 Final: BIO230 Part.2 Exam Note (with lab notes).docx

62 views24 pages

purplegrasshopper135

21 Dec 2013

School

Department

Course

Professor

For unlimited access to Study Guides, a Grade+ subscription is required.

avalack09 and 39882 others unlocked

BIO230H1 Full Course Notes

Verified Note

42 documents

Document Summary

Clathrin, copi, copii drive vesicle invagination events: rmb they transport cargos from eg. er to golgi by forming a bud on the er membrane. Signaling lipids (pips: each pi can be modified by kinase/phosphatase to generate different pips, they go to specific locations in the cell and bind with specific proteins. Rab gtpase will be brought to specific places: rab11 = recycling endosomes, rab5a = plasma membrane, clathrin-coated cesicles, early endosomes, rab7 = late endosome. Rab-gdp (with its amphoipathic helix inside) are recruited to specific membranes by rab gefs. Rab gef then exchange the gdp to gtp, activating rab gtpase (now its amphipathic hexlis is exposed). The v-snare on the vesicle (which has rab-gtp) will tether with t-snare on the target membrane. At the same time, rab effector tethering protein on the target membrane will also react with rab-gtp and bring the vesicle closer to the membrane for more efficient snare protein interaction.

Related Questions

1) Which of the following statements about transport into the mitochondria is FALSE?

a) The signal sequence on proteins destined for the mitochondria is recognized by a receptor protein in the outer membrane of the mitochondria.

b) Proteins that are transported into the mitochondria are unfolded as they are being transported.

c) Transport of proteins into the mitochondria requires SRP and the SRP receptor.

d) After a protein is made in the cytosol it must move through two protein translocators to reach the inner mitochondrial matrix.

2.Which coat protein is involved in vesicle formation during endocytosis â vesicle forms from plasma membrane and fuses with endosomes?

a) COPI
b) COPII

c) v-SNARES d) Clathrin

3. Which of the following statements about vesicular membrane fusion is TRUE?

a) Membrane fusion does not always immediately follow vesicle docking.

b) The interactions of the v-SNAREs and t-SNAREs pull the vesicle membrane and the target organelle together so that their lipids can intermix.

c) NSF and SNAP disassemble the SNARE complex so the SNAREs can be recycled.

d) All of the above are true.

The vesicles that transport materials from donor membranes to acceptor membranes include:

	1.	COP-II coated vesicles
	2.	COP-III coated vesicles
	3.	COP-I coated vesicles
	4.	Clathrin coated vesicles

2.5 points

QUESTION 11

During skeletal muscle contraction, the roles of ATP include:

	1.	ATP is not required for forming the crossbridges between myosin and actin filaments.
	2.	ATP is utilized as a structural linker to mediate the interaction between myosin and actin filaments
	3.	Hydrolysis of ATP provides energy for myosin head to move along actin filaments and the binding of ATP to myosin head allows the detachment of myosin fromactin filaments.
	4.	ATP binds to actin filaments to drive the power stroke of myosin head.

2.5 points

QUESTION 12

One of the functions of smooth endoplasmic reticulum is

	1.	Synthesizing steroid hormone in the endocrine cells of the gonad and adrenal cortex
	2.	Serves as the starting point of the biosynthetic pathway
	3.	Synthesizing secretory proteins
	4.	Degrading cell debris

2.5 points

QUESTION 13

The function of COP-II coated vesicles is mainly:

	1.	Transporting materials from endosomes to Golgi complex
	2.	Moving materials from the ER to the ERGIC and Golgi complex.
	3.	Transporting materials from Golgi complex back to endoplasmic reticulum.
	4.	Involved in receptor-mediated endocytosis.

2.5 points

QUESTION 14

Which of the following processes is completed in rough endoplasmic reticulum?

	1.	Chaperones help misfolded proteins fold correctly.
	2.	Proteins that target to nucleus are synthesized in rough endoplasmic reticulum.
	3.	O-linked glycosylation occurs in rough endoplasmic reticulum.
	4.	Misfolded proteins are destructed in rough endoplamic reticulum.

2.5 points

QUESTION 15

Kinesin is the motor protein that coorporates with microtubules. It has the following functions:

	1.	It uses GTP as energy source.
	2.	It can hydrolyze ATP and use the energy to drive cargo along microtubules from minus end to plus end.
	3.	It has a globular head that can act as GTPase
	4.	The tail of kinesin is more diverse and binds to cargo

2.5 points

QUESTION 16

For correct segregation and trafficking, lysosomal proteins are tagged in the cis-Golgi with phosphorylated mannose residues, and then the tagged lysosomal enzymes are recruited to clathrin coated vesicles by mannose 6-phosphate receptors.

True

False

2.5 points

QUESTION 17

The process that specialized cells, such macrophages, engulf relatively large particles is:

	1.	Called pinocytosis
	2.	Called phagocytosis
	3.	Mediated by COP-II coated vesicles
	4.	Called autophagy

2.5 points

QUESTION 18

Proteins synthesized on rough endoplasmic reticulum and smooth endoplasmic reticulum are imported co-translationally to their destinations

True

False

2.5 points

QUESTION 19

Treatment of cells with a drug that promotes microtubule disassembly disperses Golgi complex into separate stacks of membrane compartments, suggesting that:

	1.	Golgi complex is made of microtubules.
	2.	Microtubules are important for the organization of Golgi complex.
	3.	Microtubules are transported into Golgi complex
	4.	Golgi complex is composed of tubulin proteins

2.5 points

QUESTION 20

Which of the following is correct about signal sequence of a secretory protein?

	1.	It is an âaddress codesâ for protein trafficking pathways
	2.	It is usually located at the C-terminus.
	3.	It targets the ribosome and the protein that is being synthesized to endoplasmic reticulum.
	4.	It is removed in Golgi complex.