BCH311H1 : Tutorial questions
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Automated dna sequencing do not have four seqparate reaction vessels but four. You are given a sample of cells through to have come from a patient with a disease where prt of chormosmoe 1 has been switched with part of chromosome 5 and vice versa. Fish*** want to visualize, which chromosome switched no sites specified. Which of the following is not rue regarding solid phase nucleic acid synthesis. Nucleic acids synthesized three prime to five. Non eof the above (all above statements are correct. Five prime protecting groups need to be cleaved off after each nucleotide addition. The limit for the number of nucleotides is about eighty to a hundred kb. It is used to produce primers for pcr. Aside each step, get less and less pure dna. A researcher performs microarray analysis and feels that one of her upregulated hits are false. Which technique can she sue? fish reverse transcription/rt-pcr.
