20 - TT2 review.docx

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Molecular Genetics and Microbiology
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Alan Cochrane

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GENERAL CONCEPTS – Parvovirus replication – help melt ends and bend DNA back into T shaped structure - Autonomous part – AAV parvoviruses – inverted terminal repeates – sequences same at both ends – autonomous parvoviruses – each end has unique sequence – but sequence at one end isn’t the same as sequence at the other - Gene expression – late messengers all come from common promoter – big transcript and then splicing to make final mRNA, which will have coding sequence for protein – three little pieces of RNA, spliced onto end of each message – for getting out of nucleus and getting translated – host shut off processes disabled for most mRNA QUESTION - A cell lne has a retorviurs integrated into tis genome which has the following structure U31 R U52 _____________ U32 R U51 Virus is harvested from teh cells and used to infect a second cell line. Analysis confirmed insertion of the proviral DNA into the genome, of the following possible proviral structurs, which would you predict could be generated? a. U33 R U53 -------------- U33 R U52 b. o Transcribe the provirus – working with RNA, not DNA o Transcribe into DNA – reverse transcribe that – get only one structure U32 R U52 ----- o Answer is only U32 R U52 ----- U32 R U52 o Must follow the entire step of replication of virus - A transformed cell line has been isolated. Analysis of gene expression in the cells reveals increased expression of the myc oncogene, Further study reveals that the myc RNA conatins teh U5 and R sequences of a retroviruse. Which of the following statements is correct tregarding the nature of the transformation event induced byt eh viru? o Transformation is due to infection by a non-transducing virus via a promoter insertion mechanism ANSWER o Transformation is due to infection by a non-transducing virus via a enhancer insertion mechanism o Transformation is due to infection by a non-transducing via via a termination mechanism o Transformation is due to infection by transducing virus
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