BIOL 230- Final Exam Guide - Comprehensive Notes for the exam ( 24 pages long!)

• School: University of Victoria
• Department: Biology
• Course: BIOL 230
• Professor: Choy Francisand Barbara Ehlting

Document Summary

Genetics 230 january 9, 2018 lecture 3. In human females one oocyte is chosen every 30 day s: secondary oocyte will go into metaphase ll and is then released into the ovary. If there is a sperm to fertilize the egg the cycle will continue. Genetics 230 january 10, 2018 lecture 4. Lots of (cid:396)est(cid:396)ictio(cid:374) site so that you do(cid:374)"t cut you(cid:396) ge(cid:374)e se(cid:395)ue(cid:374)ce of i(cid:374)te(cid:396)est i(cid:374) half: allows for more variability. It can be easier if you are using 2 different restriction enzymes however it is harder to re-ligate. It depends on the mutation you make to the gene: detect introduced dna with pcr. Denature dna 2 t=strands need to separate: dna separates above 90 degrees celsius, proteins denature at 42 degrees celsius, 2. Low temperature fo(cid:396) (cid:374)ot good p(cid:396)i(cid:373)e(cid:396)s that do(cid:374)"t (cid:373)at(cid:272)h the dna exactly 60 degrees: higher temperatures is used for more exact primers, 3.