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Midterm

BIOL 351 Study Guide - Midterm Guide: Ethidium Bromide, Polyelectrolyte, Dna PolymerasePremium

4 pages67 viewsSpring 2018

Department
Biology
Course Code
BIOL 351
Professor
Beaulieu
Study Guide
Midterm

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Biology 351 Midterm #2 Questions:
1. The polyelectrolyte theory suggests that the negative charge on the backbone
of DNA is useful for what cell machinery to perform its job?
a. Nucleus
b. DNA Polymerase
c. Mitochondria
d. Plastid
2. What molecule is used for DNA visualization?
a. Ethidium Bromide
3. Describe how DNA is visualized.
a. Add Ethidium bromide to your sample, allow time to mix.
b. Ethidium bromide intercalates with the dna in between nucleotide
bases
c. Irradiate sample with UV radiation
d. Electrons in ethidium bromide are excited to a higher lying energy
level and then the excited state electrons release energy in the form of
photons, which, we observe as green light.
e. DNA is associated with the ethidium bromide molecules, therefore,
visualize dna from the emitted visible light.
4. What carbohydrate derived from seaweed is used for gel electrophoresis?
a. Agarose
5. Gel-electrophoresis seperates DNA by which characteristic(s)?
a. Size
b. Length
c. Charge Density
d. All of the above
6. What do you need for a PCR reaction?
a. Free nucleotides
b. TAQ polymerase
c. Template strand of DNA
d. Primers
e. Thermo-cycler
7. Define Gene
a. A segment of DNA, which encodes the instructions to produce a certain
protein.
8. How many genes are on a chromosome?
a. 100-low 1000s
b. 1000-10,000
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