Biochemistry 2280A Study Guide - Quiz Guide: Dna-Binding Domain, Deoxyribonuclease, Chromatography

• School: Western University
• Department: Biochemistry
• Course: Biochemistry 2280A
• Professor: Mclachlin

Document Summary

Nucleosome has crystal structure: dnase hypersensitivity & gene activation: > regions of chromatin sensitive to cleavage by the dnase i enzyme. > chromatin has lost its condensed structure, exposing the dna and making it accessible. > these accessible chromatin zones are functionally related to transcriptional activity, since this remodeled state is necessary for the binding of proteins such as transcription factors. Digest -> dnaase -> one line on chromatogram. Digest -> dnaase -> two lines on chromatogram. Nucleosomes eliminated -> active chromosomes: class ii regulatory regions: Multiple binding sites -> sequence-specific transcription factors. >dna binding domain: transcriptional stimulation -> eukaryotes. Recruitment/co-recruitment -> hats & chromatin modifier complexes: signal transduction pathway: Interaction -> ligand & receptor -> plasma membrane. Dna binding -> activation -> nuclear localisation -> degradation: activation -> transcription -> signal transduction: Protein kinase a -> binds -> creb. >> histone acetylation: creb, cyclic amp regulatory element binding factor creb. Cyclic amp regulatory element binding factor: cbp.