Biochemistry 3381A Study Guide - Quiz Guide: Plaque-Forming Unit, Vaccinia, Lytic Cycle

• School: Western University
• Department: Biochemistry
• Course: Biochemistry 3381A
• Professor: Brian Shilton

Document Summary

Strain lister serologically distinct polymerase fildelity. Further grouping :genome type hence similar replication strategies (batlimore) Historically: disease caused, transmission mode, capsid structure, immunological relatedness, protein structure, mode of replication. Smallpox = pox (variola); chickenpox = herpesvirus (varicella-zoster) Em: can"t determine infectivity: study structure diagnosis, quantification mix prep with known concentration beads. Pcr: can"t determine infectivity: known genome use primers diagnostic amplicons. Hemagglutination: can"t determine infectivity: quantitative rbc agglutination maximum dilution virus agglutinating. Antibody presence infection followed by adaptive response: not rapid, usually retrospective. Viral replication: not fission; eclipse phase: cell has no infectious virions. Eclipse: no intact virions disassembly to replicate na in cytoplasm. Latent period time to form new virions: binding, penetration + eclipse: t4 phage 20 mins, hsv 10h. Mean burst size: average virion yield/ cell (minus original infecting: specific virus / host cell combination; host cell metabolism, both t4 and hsv burst size c. 50. Binding: specific host receptors expression pattern determine tropism.