Biochemistry 3381A Study Guide - Quiz Guide: Rhodamine, Fluorescein, Antiserum
27 May 2020
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Fluorescence microscopy: general set up takes light from lower wavelength with higher energy and gives light with higher wavelength but lower energy. Electron of a flurochrome molecule can be raised to an excited state following light absorption. Fluorescence occurs when electron return to its ground state an emits light at a longer wavelength. 1st barrier filter: filter the light before. Light passes through 2 sets of filters reaching specimen only passes wavelengths that excite particular fluorescence dye. Beam-splitting mirror: reflects light below certain wavelength and transmits light above certain wavelength. 2nd barrier filter: filter light coming from specimen passes only wavelengths emitted when the dye fluorescences you only see what is fluorescent. Used to visualize the location or pattern of fluorescence in cells or tissues that have been stained with fluorescent molecules. Detect specific proteins or other molecules and tissues. Target of specific proteins, lipids, molecules : sample preparation and achievement of optimal contrast.
