Biology 2382B Midterm: Bio2382 Midterm Exam Notes

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apricotwoodchuck111

20 Oct 2011

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Light microscopes: revealing cellular structure of live stained cells. Fluorophores: when excited they kick off visible light of a different wavelength. Determine location in fixed cells, as well as localization changes in response to the environment. Light arrives at eye out of phase. Can"t use plastic slides because it interferes with polarized light. Dichroic mirror (bounces uv down to specimen and allows visible light back up) Electron microscope: look at subcellular of fixed, sectioned, cells that are not live, equation is changed! Electron microscope- revealing subcellular structures of fixed, sectioned and stained cells: transmission (tem): requires very thin (50-100 nm), fixed sections, only small part is observed, we see detail looking inside the cell (2d image). No n as light is replaced by electrons in a vacuum, very small wavelength because electrons have a short wavelength. The sample must be stained with heavy metals during or just after fixation to be seen.

Related Questions

1. How does the illumination of the specimen differ in fluorescent microscopes compared to conventional bright field microscopy? In this answer you need to explain how the filter system works in a fluorescent microscope, the light source differences if any, and the way that fluorescent molecules behave in response to light. 1b. What are some advantages of fluorescence microscopy compared to conventional bright field staining techniques?

viridianraccoon618

26. Which of the following is the best microscope to use to visualize a cross section of skin
stained so that the cells are purple and the extracellular matrix is pink:
a. Light microscope
b. Confocal microscope
c. Transmission electron microscope
d. Scanning electron microscope
e. Fluorescence microscope
27. Which of the following is the best microscope to use in order to visualize cell to cell
junctions at high magnification:
a. Light microscope
b. Confocal microscope
c. Transmission electron microscope
d. Scanning electron microscope
e. Fluorescence microscope
28. A student is studying the tensile strength of a new metallic alloy. He wants to visualize
the fracture surfaces following tensile testing. Which is the best microscope for this
application:
a. Light microscope
b. Confocal microscope
c. Transmission electron microscope
d. Scanning electron microscope
e. Fluorescence microscope
29. A graduate student is studying the degradation properties of a new polymer that she
developed. She adds a fluorescent molecule to the polymer backbone and makes a thin
layer of the polymer on a glass slide. In order to visualize the polymer and measure its
degradation/disappearance over time, which is the best microscope to use:
a. Light microscope
b. Confocal microscope
c. Transmission electron microscope
d. Scanning electron microscope
e. Fluorescence microscope
30. Endothelial cells were exposed to experimental conditions and are imaged intact under a
microscope following antibody labeling of NF-ÎºB, a protein located inside endothelial
cells. Select the following statement that best describes the approach that should be
used to image the endothelial cells in this experiment.
a. Cells are imaged under a transmission electron microscope.
b. Cells are imaged using phase-contrast in the light microscope.
c. Cells are imaged under a fluorescence microscope.
d. Cells are imaged under a scanning electron microscope.

Q: Compare and contrast the different types of microscopy?

Q: For each of the following types of light microscopes briefly describe it; state some specific uses; state some limitations of use (if applicable):

(a) Bright field

(b) Dark field

(d) Fluorescent

(e) SEM

(f) TEM

Please help. Thanks!

Biology 2382B Midterm: Bio2382 Midterm Exam Notes

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