Biology 2382B Study Guide - Final Guide: Axoneme, Dynactin, Basal Body
28 Mar 2016
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Document Summary
It looks like the microtubules are reads, and the organelles which are bound to it, are moving down the road. Axonal transport moves away from the cell body. Proteins made in the cell body are transported away, down the axon, towards the plus end. Labelled proteins travel at different speeds in cells (not diffusion) You can inject radioactive amino acids into the cell body. These radioactive amino acids will make radioactive proteins. If you wait a different periods of time and isolate different fragments of the axon. Run an sds page and determine which proteins are present in each fragment. As time progresses, you can see the banding patterns moving at different rates. Note: you have different proteins moving at different rates, in addition to those that are moving at the same rate. Kinesin, mt"s plus (+) end direction motor protein. Many types (14 known classes, 45 genes in humans)
