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Midterm

GeneticsMidterm2-QuestionSet1.docx.pdf

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Department
Biology
Course
Biology 2581B
Professor
Jim Karagiannis
Semester
Winter

Description
Sample Problem set for 281b test#2 (K Hill lectures) 1. What is the average distance in nucleotides between the restriction site in organism X for the following restriction enzyme AND what type of ends does the enzyme BamHI create after restriction endonuclease digestion? Assume an AT:CG ratio of 50:50 Recognition Sequence Bacillus BamHI amyloliquefaciens 5'---G GATCC---3' 5'GGATCC 3'CCTAGG 3'---CCTAG G---5' A. (4) nucleotides AND blunt ends B. (4) nucleotides AND 3’ sticky ends C. (4) nucleotides AND 5’ sticky ends 3 D. (43 nucleotides AND 3’ sticky ends E. (4) nucleotides AND 5’ sticky ends 2. A circular double stranded DNA molecule has three target sites for restriction enzyme AvaI. What is the maximum number of fragments that can be produced if the sample is only partly digested with AvaI? A. 1 B. 3 C. 4 D. 6 E. 7 3. You are given a sample of linear double stranded DNA. You are to determine the number and order of restriction cut sites in that DNA. You divide the DNA sample into three aliquots. In your first experiment you digest one aliquot of the DNA sample with EcoRV (RV) and after gel electrophoresis you observed three bands indicating fragments 19kb 13kb and 12kb in size. You then digest the second aliquot with EcoRI (RI) and after gel electrophoresis you observe four bands indicating fragments that are 15kb, 14kb, 9kb and 6kb in size. With the third aliquot you perform a double digest using EcoRV and EcoRI and you observe six bands indicating fragment sizes of 14kb, 8kb, 7kb, 6kb, 5kb and 4kb in size. Which is the correct map of the enzyme cut sites? A. 14kb RI 5kb RV 4kb RI 8kbRV 7kb RI 6kb B. 6kb RI 5kb RV 4kb RI 8kbRV 7kb RI 14kb C. 14kb RI 7kb RV 4kb RI 8kbRV 5kb RI 6kb D. 14kb RI 5kb RV 8kb RI 4kbRV 7kb RI 6kb E . 6kb RI 5kb RV 4kb RI 7kbRV 14kb RI 8kb 4. You are given the following vector. You attempt to insert a sequence of interest into the NheI restriction site. What is the phenotype of the host transformed with recombinant vector? A. ampR tetS B. ampS tetR C. ampS tetS D. ampR tetR E. C and D above 5. A plasmid vector has a gene for ampicillin resistance (ampR) and a gene for tetracycline resistance (tetR). The tet gene is cut with a restriction enzyme and donor DNA cut with the same enzyme is inserted in the tet gene. What phenotype of the cells needs to be selected to show evidence of transformation with the recombinant vector? A. ampR tetS B. ampS tetR C. ampS tetS D. ampR tetR E. only ampS 6. Using Southern analysis, a clone of a region of the factor IX gene from mouse is used under low-stringency conditions to probe an EcoRI digest of genomic DNA from a frog. The autoradiogram shows a single-labelled band of 4kb in size. This means that A. the frog factor IX gene is 4 kb in size B. the frog factor IX gene is the same size as the mouse gene C. the frog factor IX gene is present in four copies in the genome D. a factor IX-like gene is present in the frog genome E. the frog factor IX gene is identical in sequence with the mouse gene 7. You are making a library for an extremely large genome. Which vector would you be least likely to use? A. YAC B. BAC C. PAC D. cosmid E. lambda phage 8. After using the Sanger method for sequencing DNA (dideoxy nucleotide methods), you observe the following autoradiogram ddATP ddCTP ddGTP ddTTP From the autoradiogram, which of the following is the base sequence of the template strand: A. 5’ -TACGGGTA- 3’ B. 3’ -TACGGGTA- 5’ C. 5’ -ATGCCCAT- 3’ D. 3’ -ATGCCCAT- 5’ E. 5’ -ATGGCGAT- 3’ 9. Which of the following does base 1 to 1000 of a DNA sequence chromatogram represent? A. the sequence of the nascent DNA strand 5’ to 3’ B. the sequence of the template DNA strand 5’ to 3’ C. the sequence of the nascent DNA strand 3’ to 5’ D. the nascent sequence furthest from the sequencing primer E . the DNA strand complementary to the strand represented in an autoradiogram of a Sanger dideoxynucleotide sequencing reaction 10. Assume that a genome mapping project used
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