Biology 3593B Study Guide - Quiz Guide: Schizosaccharomyces Pombe, Mitosis, S Phase
Document Summary
Case study of genetics screens: pombe screen: nurse et al, nobel 2001. Cell cycle typical of eukaryote: g2 elongated grow until minimum length mitosis. Found cell cycle mutants: mutagenised haploid cells recessive mutation identification. Predictions: driving (forward) cell cycle essential for growth and reproduction, so ts mutants identified, regulation/mitotic progression affect division not growth elongated phenotype, no septa in mutants. Results: mutants denoted cdc: elongated, aseptate mutants: found to be recessive, also wee" mutants divide at smaller size in genes preventing fission before set length, dominant, same regulatory protein: cdc2p permissive of mitotic entry. Inactivated can"t enter mitosis: over-expression premature mitosis (wee, cerevisiae: schekman et al; nobel 2013. Growth and secretion pathway (budding: increase sa at bud need lipid, cho and protein synthesis and delivery to bud. Haploid mutagenesis: predicted: secretion essential for budding need ts mutants, secretory mutants accumulate secreted (e. g. acid phosphatase)in cell, impede growth use acid phosphatase as marker.
