Biology 3593B Study Guide - Quiz Guide: Retinitis Pigmentosa, Drug Test, Huntingtin
Document Summary
Fermentable (glucose) c source grow w/out. Non-fermentable (glycerol) essential must respire: easy to isolate essential lof genes- condition sensitive genes. Mt dna encoded atp6 mutants nervous system symptoms: maternal transmission. Hence, yeast atp6 mutated: poor growth on glycerol low atp production must mitochondrially respire. Screened 12k compounds for efficacy ch supressed effect of atp6 mutation in some alleles further studied. When less conservation: express human gene in yeast (hr) Need to identify phenotype related to original disease then effect of genes and chemicals. Alzheimer"s: amyloid (a ) insoluble plaques in brain. Cleaved from transmembrane app (a precursor protein: secreted. Yeast model a 1-42 fused to signal peptide secrete: under regulatable promoter expression > toxicity. Inserted in yeast overexpression library strong regulatable promoter screen for dosage suppression: 40 modifiers 12 w/ higher eukaryotic homologues, yap1802 human picalm clathrin cage and endocytosis strong. Drug screen of fusion protein yeast 140,000 small molecules assayed.
