Biology 3594A Midterm: Genetic Testing Tutorial 1 Notes
22 Dec 2011
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Sequencing is expensive and can"t be used for low cost testing methods. Minisatellite - bigger, 50-150 bp in size, core + variable (2 regions) - this makes up one region and that is what would get amplified over and over again, too big to amplify. Microsatellite - smaller, 2-3 bp, ie. ca in repeat. Three possible solutions: pcr and re digest, then gel electrophoresis, pasa (pcr amplification of specific alleles) - you get into a scenario where one of your primers is overlapping the snp area of interest. Allele 1 is exactly complimentary to primers a and b. allele 2 mismatches primer fwd near 3" end. Won"t get a strand the gel for the later method because the strands do not anneal with the snp: bi-pasa (bi-directional pasa) - instead of having two primers, we have four primers. Two of them are flanking the area of interest (where the nucleotide polymorphisms are) and they are normal forward and reverse primers.
