Biology 1002B Study Guide - Final Guide: Sanger Sequencing, Functional Genomics, Start Codon
16 May 2016
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Document Summary
Sanger method is based on the properties of nucleotides known as dideoxyribonucleotides: have a single h bound to the 3" carbon of the deoxyribose sugar instead of the oh normally at this position in deoxyribonucleotides. Drn; base at 3" end is known and due to base-pairing rules, the base on the template strand being sequenced can be deduced. In whole genome shotgun method, the entire genome is broken into thousands to millions of random, overlapping fragments which are clone and sequenced: it is then assembled by computer on the basis of sequence overlaps between fragments. Annotation is the identification of genes and other sequences of importance. Bioinformatics fuses biology with maths and computer science: also used to predict the structure and function of gen products and to postulate evolutionary relationships of sequences. Genomes widely vary in size dramatically within a domain.
