Biology 3355A/B Study Guide - Final Guide: Uridine, Organogenesis, Nuclear Localization Sequence

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Base excision repair: dna glycosylase recognizes t-g mismatch and flips it out of helix, creating an ap site, ap endonuclease recruited to cut around mismatch, ap lyase removes deoxyribose-phosphate, dna polymerase beta fills in space + ligase seals. Mismatch repair: msh2 and msh6 recognize non-t-g mismatch, endonuclease cuts it off, helicase unwinds helix, exonuclease cuts edges, dna polymerase delta + ligase. Nonhomologous end joining: ku70/80 recognizes ds break and recruits dna-pkcs, other proteins recruited to trim ends, ligase to seal strand. Also nuclear export signal and nuclear localization signals. Nothing bound to car, then ring finger can interact with p53 binding domain so p53 is induced to e3 ubiquitin ligase for degradation. Arf and rps bind to car, ring finger can"t conformationally distort itself to interact p53 pathway. Stress response of nucleolus: p53 activation: rp binds to mdm2 in nucleus so p53 is active, rpl26 acts as cofactor in stimulating p53 mrna, rpl11 moves rps into nucleus for 1.