CH356 Study Guide - Midterm Guide: Molecular Cloning, Recombinant Dna, Cas9
Document Summary
There are 4 key principles and 4 key applications in bioethics: Dna sequencing: unmask the tendency to acquire a genetic disease. The individual can make lifestyle changes earlier to avoid disease. The individual can apply for genetic treatment for such a disease. Earlier detection can possibly help in psychological adjustments. The genetic data can be used by others for nefarious purposes. If not guarded properly, the genetic data can become public. Insurance companies may use the genetic data against the owner. Dna cloning: separating a specific gene (or dna segment) from a larger chromosome restriction enzymes, attaching dna to a carrier cloning vector, replicating (multiplying) modified dna in host cell recombinant dna. Restriction enzymes: endonucleases cleaves nucleic acids. Nucleases: cleaves nucleic acids, exonucleases p1 nucleases ssdna. Cleave dna sequence specifically: 4-8 bp in double stranded dna, usually palindromes, cleaves phosphodiester bonds. Restriction sites: sticky ends, blunt ends.
