BIOL 2021 Study Guide - Midterm Guide: Extracellular Fluid, Choline, Phagocyte
Document Summary
Regulation of vesicle formation: 13-14: lipid marker: phosphinositides, coat recruitment gtpases: Gef: guanine nucleotide exchange factor is active. Arf protein: active in cop1 coat and clathrin coat at golgi. Inactive sar1-gdp active: sar1-gep in membrane sar1 binds to gtp and changes conformation and binds to membrane. Cop2 vesicle formation from sec23 and sec24 proteins to bind sar1 and cargo receptors bind to adaptor proteins = inner coatore coat proteins bind which make a curvature = outer coat proteins. Uncoating: sar1 gtp hydrolyzes sar1 gdp + pi tail pops out of membrane and everything that was bound falls off, coat is lost, sar1 gtpase is on a timer. Identity marker for organelles both donor and target membranes. Bind to rab effector proteins on opposite membrane, specific target, vesicle gets docked/tethered on a membrane tethering (tieing a rope to a post) Fusion uses snares: v-snare: on vesicle, t-snare: on target.
