BIOL 311 Study Guide - Midterm Guide: Förster Resonance Energy Transfer, Fluorescence In Situ Hybridization, Photobleaching
11 Mar 2018
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Transgenic cells: knock in / knock out cells. Can be used to determine if two proteins interact in vivo. Can be used to determine the diffusion coefficient of membrane proteins. Can be used to perform chromosome painting which helps in differentiating chromosomes of similar size and shape. Can be used to separate cells that are labeled differently with a fluorescence reagent. Northern blots: separates and identifies select rnas in a gel. Southern blots: can detect a single gene copy: do not require the use of a monoclonal antibody: hybridization, southern blotting combines transfer of electrophoresis-separated dna fragments to a filter membrane and subsequent fragment detection by probe hybridization. Western blots technique: identifies a specific protein based on the affinity of a specific antibody: gel electrophoresis, blot or a transfer of proteins to a filter paper, antibodies, quantitative and qualitative. Epigenics: the study of changes in organisms caused by modification of gene expression.
