BIOL 401 Study Guide - Midterm Guide: Ribosome-Binding Site, Rna Polymerase Iii, Dna-Binding Domain

• School: Binghamton University
• Department: Biology
• Course: BIOL 401
• Professor: Steven P Tammariello

Document Summary

Taq polymerase: heat stable, comes from an organism that lives at very high temperatures, allows pcr without having to add dna polymerase every round. If the temp was lowered to 55, random annealing: add more salt to allow annealing in more places. Pcr cycle: exponential, plateau phase (undesirable in pcr because it can yield inaccurate results) so much dna, not enough primers, after about 40 cycles, runout of primers and dntps are mostly used up. It took longer for the blue ones to reach certain points so they must have less dna. Taqman probe: works extremely well, primers pointing each way, and then probe sits down somewhere in the middle of the. If no ddntp then the whole sequence would be synthesized: put in template you want to sequence, primer, polymerase, dntps (all four) Automated sanger the gold standard: pros: accurate, read length about 400 bp, cons: high cost, relatively low throughput.