MCDB 2150 Midterm: Problem Set 4 Homework Review
2 Mar 2017
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Rnai: a researcher generated small interfering rnas (sirna) against rna polymerase. Ii and treated the worm embryos, and then observed the result. In this experiment the transcription of the embryonic genome was successfully inhibited by the sirnas. The sirna bound to the rna transcript for rna polymerase, preventing. Gene regulation it from being translated: mutation that causes normal sized mrna, normal sized protein, but reduced amounts of both: Mutation in a large intron that contains a regulatory element. A mutation in an enhancer element upstream of the transcription start site: most likely an effect of a mutation in the 3" utr: Sequences can be aligned by looking for a reference sequence that is found in most of the fragments. Then, fragments that contain that reference sequence can be aligned based on additional similar sequences they contain. As each new fragment is added, the contig (and contiguous sequence) gets longer.
