BSC 4930 Study Guide - Midterm Guide: Alternative Splicing, Rna Interference, Antennapedia
Document Summary
Microscopy: confocal lets you look at a specific focal plane. Fluorescence: colors to tag different things; gene expression,proteins. Genetics well understood/known: big 6: match common and scientific and advantages. Control of gene expression- different levels: regulatory proteins, transcription factors. Specific: tissue-specific, spatial and temporal regulation; where and. Acetylation- more open conformation- leading to increased transcription: far less acetylation in barr bodies. Rna level: post transcriptional: rna interference. Different splice sites in different tissue types- creates isoforms of different types of proteins. Protein degradation: tagged with ubiquitin sends them to proteasome to be degraded: rate of protein degradation can impact gene expression. Common: stimulation of growth, induction of apoptosis, change in gene expression of target cell. Membrane receptors: g protein (g protein, tyrosine kinases), ligand-gated ion channels (channels change shape) Protein kinases: transfer phosphates from atp protein phosphorylation. Notch mutants: lof leads to excessive overgrowth of nervous tissue: ligands: delta and serrate, lateral inhibition- paracrine.
