MCB 3020 Study Guide - Quiz Guide: Catabolite Repression, Trp Operon, Protein Splicing
Document Summary
Quiz 6 review: describe the structure of bacterial chromosomes. Generally negatively supercoiled (coiled in a way to decrease basepairs/turn: know the key type of sequences in a promoter that are usually constant or similar in different bacteria. Site where rna poly binds to initiate transcription; not transcribed. Pribnow box (consensus sequence)- tataat- 10 bp upstream -10. Ttgaca sequence 35 bp upstream -35: understand the concept of the genetic code for amino acids. Code degeneracy- up to six different codons can code for a single amino acid. Wobble- 3rd position less important than 1st or 3nd- eliminates need for unique trna for each codon: know the regions of the operon that are transcribed and translated. Replicon: portion of genome that containts an origin that is replicated as a unit. Trailer (immediately after stop codon) is transcribed, not translated. Transcription start site that is transcribed but not translated. Ribosomal subunits: 50s and 30s in prokaryotes, 60s and 40s in eukaryotes.
