BSC 1010C Study Guide - Quiz Guide: Sanger Sequencing, Dna Profiling, Plasmid
Document Summary
The manipulation of organisms or their components to make useful products using whole organisms but also, cell and tissue culture technologies. An enzyme that cuts dna: describe how they act on dna. The sticky" end can glue new dna: how can they be used to reveal differences between two different samples of dna, what are genomic libraries and give two examples of how they are stored. Two examples: plasmid library and phage library: describe how the polymerase chain reaction (pcr) technique is able to amplify (copy) a small sample of dna into a large sample in just a matter of hours. Original dna template strand is used to create a set of dna strands complimentary to the original fragment. Chain-elongating inhibitors of dna polymerase, used in the sanger method for dna sequencing. Fragment synthesis stops when dna polymerase meets deoxy.
