MICR 803 Study Guide - Quiz Guide: Dna Replication, Okazaki Fragments, Dna Ligase
Document Summary
Dna replication: identical copies of their dna before cell division, binary fission. Double stranded dna: two strands on nucleotides joined by hydrogen bonding deoxyribose sugar and phosphate backbone made of phosphodiester bonds. A-t forms 2 hydrogen bonds, takes less energy to break. Two template strands used to create two new molcules, adding nucleotides to the 3 prime end. Dna replication begins at the origin of replication, active area of replication called the replication fork. Single stranded binding proteins stabilize the single stranded helix. Dna gyrase the double stranded areas outside the replication fork don"t supercoil. Dna polymerase 3 adds nucleotides to growing daughter strands. Beta clamps and clamp holder help hold the dna poylmerase on the dna. Short sequences of rna (primers) are added to the strand by dna primase. Dna polymerase cannot add nucleotides without a primer. Continuous synthesis on the leading strand, moving towards replcation fork adding to the 3 prime end.