BIOL 2083 : Long Answer Exam 4
Document Summary
Possible long answer questions: describe the structure of the pyruvate dehydrogenase complex and how it contributes to efficient function (describe relative locations of enzymes and relevant cofactors). The three enzymes of the pyruvate dehydrogenase complex are structurally integrated, and the lipoamide arm allows rapid movement of substrates and products from one active site of the complex to another. The pyruvate dehydrogenase complex, a mitochondrial matrix enzyme, oxidatively decarboxylates pyruvate to form acetyl coa. The cofactors are attached to the enzymes: the structure of pyruvate dehydrogenase complex consist of three enzymes: e1,e2, and e3. E1 is the enzyme that performs the oxidative decarboxylation of pyruvate, e2 performs the transfer to coa and e3 regenerates the cofactors in order to reset the cycle. Stoichiometric cofactors which consist of : coa and nad+, these enzymes are equivalent to a substrate and are consumed during the reaction. Enzyme e1 is a key site of regulation.
