GENE 540 Study Guide - Midterm Guide: Hydroperoxyl, Cellular Respiration, Uracil
Document Summary
Uv light, x-ray, chemicals these can all generate ros. But since you have to remove a hydrogen from the unsaturated lipid to get the lipid peroxide, you have just created another lipid radical the cycle keeps going. Hence, even a little bit of free radicals can cause a lot of damage. Direct production from uv light and ionizing radiation. Certain chemicals may redox cycle with subsequent production of ros. Peroxisomes: oxidative metabolism generates some ros (mainly h2o2) Deamination of cytosine (hydrolyzing the nitrogen bond) this will create. Uracil base is an rna base, so the dna repair systems should be able to recognize it and prevent its insertion into the dna. However, if its mutated in the strand, then it has to be repaired prior to. Cytosine is a dna base, so if its methylated and then it goes through deamination reaction, it creates. Thymine, then the daughter strand will have that mutation. Base-excision repair helps fix base mismatch ex.
