BIOL 1122 Study Guide - Final Guide: Bacitracin, Postpartum Infections, Human Microbiota
3 May 2017
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Y7 nutrient broth: complex-type medium, don"t know exact nature of carbon sources. Glucose-salt broth: chemically defined medium since the exact nature and amount of each component is known. Good for observing cellular morphology (shape) and arrangement. Shapes: spherical (cocci), cylindrical (rods and bacilli), curved (coma or vibrio), squared, and spiral (spirllium) Arrangements: clusters (staph), chains (diplococci, streptococci and streptobacilli), packs of 4 or 8, and individually. Gelatinous material exterior to cell wall made of polysaccharides and polypeptides. Virulence factor: invasiveness of host tissues- avoid ingestion by neutrophils and macrophages of immune system. Negative staining: maneval stain - stains the cells and background but not the capsule. Can survive harsh environmental conditions for long periods - regarded for survival. Gram stains and simple stains fail to stain the spore. Spore malachite green staining - heat smear while applying malachite green, rinse with water: cells and sporangia lose dye will spores retain it.
