14:440:301 Study Guide - Elution, Analyte, High-Performance Liquid Chromatography

• School: Rutgers University
• Department: General Engineering
• Course: 14:440:301
• Professor: All

Document Summary

Dead volume tailing: spaces before the column, tubings too big in diameter, too much space. Gc: the gas is the mobile phase, basically a box, have an oven inside rows of tubing (column separation) that is placed inside the rack of the oven adjust temperature to 350 degrees celsius. Injector at 350 degrees, detector at 300 degrees introduced into the system must be in vapor phase: at the very beginning see the carrier gas go to the column vent (split vent at. 300 until everthing comes off the column: detector has to be at high temperature as well because you want everything that comes off to also be in vapor phase. Column: using open tubular column 90-95% of the time, can use packed column but not used as common, otc very small diameter 0. 1- 0. 53 inner diameter in this there is a layer of sp.