BIO 463 Study Guide - Hybridization Probe, Deoxyribonuclease, Anaphylaxis
Document Summary
Chp 7: eukaryotic expression: glycosylation difference: glycosylation is the addition of specific sugars to certain amino acids to provide stability and distinctive binding properties to a protein. Oligosaccharides are attached to newly synthesized proteins in the endoplasmic reticulum and in the golgi apparatus by specific enzymes known as glycosylases and glycosyltransferases. a. b. O glycosylation- attachment to specific sugars to hydroxyl group either serine or threonine. O-linked oligosaccharides have a number of arrangements with different combinations of sugars. N glycosylation- attachment of specific sugar to amide group of asparagine. Denote inherited changes in animal cells that are due to addition of (forgein. Dna: general understanding of eukaryotic expression vector, must have eukaryotic promoter that drives the transcription of the cloned gene of interest, eukaryotic transcriptional and translational stop signals. They have growth advatnages similar to those of prokyarotes. 2 um vector a small, circular plasmid found in the most natural strains of s. cerevisiase.
