CELL 1010- Final Exam Guide - Comprehensive Notes for the exam ( 35 pages long!)

233 views35 pages

Document Summary

Concept 4. 1 - biologists use microscopes and the tools of. Cells were first visualized by robert hooke in 1665 due to the invention of the lens by anton van leeuwenhoek. Light microscopes pass visible light through a specimen and then through a glass lens to increase the magnification of the subject. Magnification: the ratio of a(cid:374) o(cid:271)je(cid:272)t"s i(cid:373)age to the a(cid:272)tual size of the object. Resolution: the measure of the clarity of an image. Contrast: the difference between the brightness and darkness of an image. To study organelles, which were previously unable to be seen due to resolution inadequacies, electron microscopes were developed. Electron microscopes (em) focus a beam of electronic light onto a subject and the refraction of the wavelength produce a much greater resolution than light microscopy. Scanning electron microscopes (sem), which scans the amount of electronic excitement produced by the beam on a gold-coated sample, is useful for detecting the topography of samples.