BSC 444 Study Guide - Midterm Guide: Capsid, Recombinant Virus, Viral Pathogenesis
6 Sep 2017
School
Department
Course
Professor
Document Summary
Most of the host"s defenses against viruses are not finely tuned- instead we use a. Lecture 3: virus classification, nomenclature and transmission: what is the basis for the baltimore classification scheme and what are the 7. Ii- ssdna viruses, (+)dna or (-)dna +/- dsdna mrna. Virus stock titration (1): 50% tissue culture infective dose assay: good points: scoring or it is easy and quick, bad points: does not give an exact number. Virus stock titration (2): qpcr: good points: people who don"t have to deal with tissue culture, no plating, bad points: expensive, not going to get an accurate titer (concentration, quantify genomes not infectious particles. Virus stock titration: serial dilution + plaque assay: good points: more accurate. One-step (single-step) growth curve: measures relative burst of a particular virus under particular conditions, assay at various times after a synchronous infection to obtain relative virus yields over.
