BIL 250 Study Guide - Midterm Guide: Genetic Screen, Map, Dna Profiling
15 Nov 2016
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Physical map: physically identifiable regions w/o recombination analysis. Relative to centromere (numbered starting with 1) q- long arm vs. p- short arm. Physical map of overall structure of each chromosome. Entire genome or single chrom can be represented. Overlapping clones assembled into scaffold w/o sequencing by dna fingerprinting. Locating: easy if gene product/protein is identified: create cdna library with expression vector, probe with antibodies that bind gene product, isolate & sequence positive clones. Strong stat assoc w/ disease phenotype in test crosses or genome-wide association study (gwas) Use positional cloning + chromosome walking to sequence. Positional cloning used for identifying cf gene. Most common lethal genetic disease in the us (1/2000) Thick mucus secretions in the lungs, pancreas, and intestines. Causes lung disease and organ failure, chronic bacterial inf. Single recurring rflp weak linkage to cf trait. Found cf locus within 500,000 bp regioin of chr 7. Don"t (cid:449)o(cid:396)k (cid:449)ell (cid:449)ith highly (cid:396)epetiti(cid:448)e, scatte(cid:396)ed se(cid:395)uence.
