BY 123 Study Guide - Quiz Guide: Dna Gyrase, Semiconservative Replication, Okazaki Fragments
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Compare and contrast dna polymerase and rna polymerase. Need 3" -oh to extend a dna strand. Proteins that synthesize new strands of nucleotides (nucleic acid) A new nucleotide can only be added to a free -oh group on the 3" carbon. Thus, a new strand grows in the 5" to 3" direction. Draw double-stranded dna forming a replication bubble. Indicate 5" and 3" ends of both strands of dna, the origin of replication, and the 2 leading strands and the 2 lagging. Using the diagram you drew for question 4, explain why the lagging strand is the strand with okazaki fragments. Because dna strands only grow 5" to 3", only one side of the replication fork can smoothly follow helicase. The other side needs to wait until primers are laid down and each to be elongated towards the replication fork. Okazaki fragments and the strand is lagging because of all the waits and extra steps of primers and ligation.
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