BCH 100 Study Guide - Final Guide: Triosephosphate Isomerase, Isomerase, Pyruvic Acid

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Lecture 10: glycolysis: terminology (explain/define; understand use of terms): (cid:862)i(cid:374)vest(cid:373)e(cid:374)t(cid:863) phase, (cid:862)pay-off(cid:863) phase, substrate-level phosphorylation. Generate 4 atp (net gain of 2 atp) Adding phosphate directly, without atp: state in which steps in glycolysis atp is invested and in which steps atp is produced. Atp is invested in the 1st and 3rd steps: Atp is produced in the 7th and 10th steps: The 3rd step is the first committed step to continue the glycolytic pathway. Pfk-1 is the enzyme that catalyzes the first committed step. 2 hormones help regulate: glucagon and insulin. Must shut down glycolysis, export glucose to blood to tissues. Glucagon decrease pfk-2 activity less f-2,6-bp decrease pfk-1 activity slow down glycolysis. Must increase glycolysis (or store glucose as glycogen), remove glucose from blood into tissues. Write the anaerobic reactions (substrates, products, names of enzyme) for lactate fermentation and for conversion of pyruvate to ethanol (2 reactions).

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