LIFESCI 3 Study Guide - Midterm Guide: Lac Operon, Lac Repressor, Trp Operon
Document Summary
Questions 1-4: use the codon table in the appendix to answer the following multiple-choice questions. N-term met-trp-met-his-arg c-term: 5"- atgtggatgcatcgc -3", 5"- atgtggatgcacgaa -3", 5"- atgtggatgcaccgt -3", a. and b, a. and c. Questions 5-15: use what you have learned in lecture to answer the following multiple-choice questions. Circle only one answer: an oligo-dt column is a type of affinity chromatography whose medium are beads that contain synthetic poly-t nucleotides chains hanging off the bead surfaces. This can be used to attract and separate specific molecules in a crude sample. Md, ph. d, performs an experiment to determine the effects of operator mutation on the lac operon and its regulation. He uses site-direction mutagenesis to induce a mutation in the lac operator in an e. coli cell; this prevents the binding of protein to the operator sequence. He then treats this: coli cell with different combinations of lactose and glucose.
