MCB 150 Study Guide - Midterm Guide: Cell Membrane, Cell Nucleus, Protease

• School: University of Illinois
• Department: Molecular and Cell Biology
• Course: MCB 150
• Professor: Brad Mehrtens

Document Summary

Describe the overall structure of the nucleus. Beads on a string inside nucleoplasm - chromatin. Space between the outer and inner membranes. Outline how fluorescence can be used to demonstrate chromosomal territories in interphase nuclei. Need to be denatured in order to hybridize fluorescent label. Add probe molecules to the immobilized and denatured dna on the slide. Will hybridize to the correct sequence that we made. Overlapping probes for different parts of the chromosomes. Can paint each chromosome a different color. Fish and chromosome painting are on m phase chromosomes. Chromosome painting on interphase chromosomes shows that the nucleus is divided into domains. Describe the organization of replication, transcription, and rna processing into functional nuclear domains. This allows us to visualize the oris. Location of fluorescence = location of replication. We would not see localized regions of color. It would be a light spread of color. We would see localized locations of color.