BIOLOGY 285 Study Guide - Final Guide: Thymine, Regulatory Sequence, Covalent Bond
5 Dec 2012
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Chargaff"s rule: a:t, g:c in approximately 1:1 ratio for every organism. Dna = base + sugar + phosphate back bone. 2" deoxyribose sugar; has h at 2" position instead. Oh in rna is crucial because it is used as atp to synthesize. Bases interact through van der waals and h-bonds. Decrease salt concentration because backbone is negatively charged . Cutting both strands in half because the hydrogen bonds (2kcal each) are easier to break this way. Restriction endonuclease used to attack virus dna. Restriction endonuclease requires h2o. ratp: (aka atp): datp: Nucleotides are added at the 3" end of dna. Free 3"oh attacks free 5" phosphate yields pyrophosphate and phosphodiester bond. Planar: the dna stacks upon each other, rigid, w/slight offset angle. On same strand, they interact through van der waals interactions. The bases interact through complementary base pairing through hydrogen bonds on opposite strands. Pyrophosphate: high in e drives bonding cannot go backwards.
