BIOL 367 Chapter Notes - Chapter 11: Peptide, Ubtf, Ribonuclease
Document Summary
Chapter 11 general transcription factors in eukaryotes. General transcription factors combine with rna polymerase to form a preinitiation complex. Involves formation of an open promoter complex in which the dna at the transcription start site has melted to allow the polymerase to read it. The class ii preinitiation complex contains polymerase ii and 6 general transcription factors named tffia, tfiib,tfiid, tfiie, tfiif, and tfiih. They bind in a specific order to the growing preinitiation complex, at least in vitro. This experiment reveals the existence of 4 distinct complexes, which are labeled at the left of the figure. When the investigators added tfiid and a alone to dna containing the adenovirus major late promoter, a da complex formed (lane 1) When they added tfiib in addition to d and a, Central part of figure shows when the yadded various concentrations of rna polymerase ii andtfiif to the dab complex. polymerase no difference was detectable.