BIOL-2011EL Chapter Notes - Chapter 8: Recbcd, Microbiological Culture, Reca

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Copyright © 2014 Pearson Education, Inc.
Essentials of Genetics, 8e (Klug)
Chapter 8 Genetic Analysis and Mapping in Bacteria and Bacteriophages
1) Name the general category into which double-stranded circular extrachromosomal DNA
elements such as F factors, ColE1, and R would fall.
A) capsid
B) r-determinant
C) plaque
D) partial diploid
E) plasmid
Answer: E
Section: 8.4
2) Bacteriophages engage in two interactive cycles with bacteria. What are these cycles?
A) lytic and lysogenic
B) insertion and replication
C) auxotrophic and prototrophic
D) heteroduplex and homoduplex
E) negative and positive
Answer: A
Section: 8.6
3) A bacteriophage that is capable of entering either a lytic or lysogenic cycle is called a(an)
________.
A) temperate bacteriophage
B) virulent bacteriophage
C) plasmid
D) episome
E) plaque-forming unit
Answer: A
Section: 8.6
4) The clearing made by bacteriophages in a "lawn" of bacteria on an agar plate is called a
________.
A) clear zone
B) lysogenic zone
C) prophage
D) plaque
E) host range
Answer: D
Section: 8.6
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Copyright © 2014 Pearson Education, Inc.
5) Temperate phages are those that can enter either the ________ or ________ cycle.
A) lytic; lysogenic
B) virulent; avirulent
C) functional; nonfunctional
D) former; nonformer
E) complementing; competing
Answer: A
Section: 8.6
6) Name two forms of recombination in bacteria.
A) lytic and lysogenic
B) auxotrophic and prototrophic
C) conjugation and transduction
D) mixed and generalized
E) insertion and replication
Answer: C
Section: 8.2, 8.5, 8.7
7) Transduction is a form of recombination in bacteria that involves ________.
A) 5-bromouracil
B) F plasmids
C) bacteriophages
D) fertility factors
E) physical contact between the bacteria involved
Answer: C
Section: 8.7
8) How does an auxotroph differ from a prototroph?
Answer: Auxotrophs have lost, through mutation, the ability to grow on minimal medium.
Prototrophs are capable of growth on minimal medium.
Section: 8.1
9) What are prototrophs?
Answer: Prototrophs are bacteria that can grow on minimal medium and are assumed to be wild
type.
Section: 8.1
10) In general, what two methods are used to grow bacteria in the laboratory?
Answer: liquid and semisolid (agar) media
Section: 8.1
11) Name the typical phases of the bacterial growth cycle in liquid culture medium.
Answer: lag, log (exponential), and stationary
Section: 8.1
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Document Summary

Chapter 8 genetic analysis and mapping in bacteria and bacteriophages: name the general category into which double-stranded circular extrachromosomal dna elements such as f factors, cole1, and r would fall, capsid, r-determinant, plaque, partial diploid, plasmid. Section: 8. 4: bacteriophages engage in two interactive cycles with bacteria. What are these cycles: lytic and lysogenic, insertion and replication, auxotrophic and prototrophic, heteroduplex and homoduplex, negative and positive. Section: 8. 6: a bacteriophage that is capable of entering either a lytic or lysogenic cycle is called a(an) ________: temperate bacteriophage, virulent bacteriophage, plasmid, episome, plaque-forming unit. Section: 8. 6: the clearing made by bacteriophages in a lawn of bacteria on an agar plate is called a. ________: clear zone, lysogenic zone, prophage, plaque, host range. 1: temperate phages are those that can enter either the ________ or ________ cycle, lytic; lysogenic, virulent; avirulent, functional; nonfunctional, former; nonformer, complementing; competing.

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