HLSC-124 Chapter Notes - Chapter 4: Hans Christian Gram, Crystal Violet, Gram Staining
22 Sep 2016
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Hlsc 124: lecture notes+ supplemental textbook notes chapter. Tortora, g. , funke, b. , & case, c. (2016). Staining: colouring a microorganism so that its different structures are emphasized and more easily studied. Smear: fixing a microorganism to a slide while simultaneously killing it. Generally, the slide is fixed through heating the sample and allowing it to air dry. Bacteria are negatively charged organisms when in a neutral ph: two types of stains, but only basic stain will work on bacteria, cannot use acidic stain see questions. Simple stains: will show you shape of a cell but not well enough to identify it, shows arrangement and size, a mordant can be used to intensify colour or coat a structure so it is more easily observed. Iodine i2 with crystal violet: basic stains: Dye the background instead of the organism. Differential stains: react differently when they come in contact with different types of organisms, hans christian gram.
