BIOL 2050 Chapter Notes - Chapter 14, 15.1: Restriction Fragment Length Polymorphism, Plasmid, Zinc Finger Nuclease
Document Summary
14. 1 molecular techniques are used to isolate, recombine, and amplify genes. Recombinant dna technology: set of molecular techniques for locating, isolating, altering and studying dna segments, goal is to combine dna from 2 distinct sources, called genetic engineering. Before, info about the structure and organization of genes was gained by examining their phenotypic effects, but molecular genetic analysis allows the nucleotide sequences themselves to be read. This provided new info about the structure and function of genes and has altered many fundamental concepts of genetics. The biotechnology industry has grown around the use of recombinant dna technology to develop new products. Imagine we want to use bacteria to produce large amounts of a human protein. First problem is to find the gene that encodes the desired protein. If the scientist succeeds in locating and isolating the desired gene, they next need to insert it into a bacterial cell.
