MBG 1000 Chapter Notes - Chapter 19: Genetically Modified Organism, Dna Replication, Electroporation

• School: University of Guelph
• Department: Molecular Biology and Genetics
• Course: MBG 1000
• Professor: Christine Schisler

Document Summary

Biotechnology is the use or modification of cells or biological molecules for a specific application. Dna patenting is evolving to embrace genome-wide applications. Pcr is based on dna replication and has many uses. In recombinant dna technology, a cell receives a cloning vector that contains foreign. Genes are isolated from genomic dna libraries or cdna libraries. Antibiotic resistance genes and gene variants that change the colour of growth media are used to select cells bearing recombinant plasmids. Recombinant dna technology is used to manufacture large amounts of a pure protein in single cells and to create multicellular transgenic organisms. Transgenic animals receive foreign dna naked, in liposomes, or by electroporation, microinjection or particle bombardment. The gene must be transcribed and translated and its products collected and purified. Bioremediation uses natural abilities to detoxify environmental contaminations including genetic modification and experimental evolution. Dna microarrays enable researchers to track gene expressions.