BIOC 202 Chapter Notes - Chapter 8: Glycolysis, Pyruvic Acid, Ribose

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2 Feb 2019
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Rxn #1: glucose phosphorylated using atp by hexokinase on c6 yielding glucose 6 phosphate, this traps glucose in cell, charged group, can"t diffuse back across membrane, destabilizes glucose. Rxn #2: glucose 6 phosphate converted -->fructose 6 phosphate by phosphoglucose isomerase, multistep process, glucose 6 phosphate->open chain->fructose 6 phosphate(open chain)->cyclic furanose, reversible rxn. Rxn #3: phosphofructokinase (pfk1) phosphorylates fructose 6 phosphate -->fructose 1,6 bisphosphate, key regulatory enzyme, is committed step. Rxn #4: aldolase cleaves fructose 1,6 bisphosphate -->dihydroxyactone phosphate (dha) and glyceraldehyde 3 phosphate (gap, reversible, yields 2 3c fragments: 1 ketose (dihydroxyacetone phosphate), 1 aldose (glyceraldehyde 3 phosphate, but only want glyceraldehyde 3 phosphate (gap), not dha. Rxn #5: triose phosphate isomerase turns dhap-->gap, reversible. Inorganic phosphate can attack carbonyl, cleaves rxn intermediate from cys, product is formed. Rxn #7: 1,3 bpg phosphorylates adp, form atp and 3 phosphoglycerate, done by phosphoglycerate kinase, an eg of substrate level phosphorylation.

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